Usefulness of Arbitrarily Primed Polymerase Chain Reaction (AP-PCR) Method for DNA Fingerprinting Analysis of Mycobacterium tuberculosis (MTB); Comparison between DNA Restriction Fragment-Length Polymorphism (RFLP) Method and AP-PCR Method
نویسندگان
چکیده
منابع مشابه
DNA fingerprinting of mammalian cell lines using nonradioactive arbitrarily primed PCR (AP-PCR).
probe. Retention of the probe on the dialysis bag using the electroelution protocol and inefficient binding of the probe to glass beads (as seen with ds DNA below 0.5 kb in size) contribute to probe losses. Since the ss probe does not need to be purified from the LMP agarose gel, loss of the probe associated with the two established protocols described here (particularly with the glass bead pro...
متن کاملPolymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for rapid diagnosis of neonatal sepsis
BACKGROUND & OBJECTIVES The difficulties in diagnosis of neonatal sepsis are due to varied clinical presentation, low sensitivity of blood culture which is considered the gold standard and empirical antibiotic usage affecting the outcome of results. Though polymerase chain reaction (PCR) based detection of bacterial 16S rRNA gene has been reported earlier, this does not provide identification o...
متن کاملAssociation between Insulin–Like Growth Factor I Polymorphism and Early Growth Traits in Iranian Zandi Sheep, Found Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP)
Early growth traits in sheep are economically important. Breeding for these traits has been shown to be problematic using quantitative genetic methods, particularly in native sheep herds. Molecular genetics is useful for sheep breeding. The gene for insulin-like growth factor 1 (IGF1) is one of the important candidate genes known for ovine early growth traits. The aim of this study was to inves...
متن کاملKey words: arbitrarily-primed polymerase chain reaction (AP-PCR), methicillin-resistant StaNtylococcus aureus (MRSA)
要 旨 MRSAに よる院内感 染症 は現 在社 会問題 に もな ってお り,そ の対 策 は重要 な課 題 であ る.院 内感 染症 の証明 にはMRSAの 正 確 なtypingが 必 要 不可 欠で あ る.し か しなが ら,近 年 コ アグ ラーゼII型 の 占め る割合 が増 加 し,コ アグ ラーゼ型 別 に よるtypingは 困難 とな って きて い る.今 回我々 は当科入 院 中の患 者 よ り分 離 され たMRSA株 に対 し,新 た なtyping法 と し てarbitrarily-primed polymerase chain reaction (AP-PCR)法 を試 み,こ のPCRに 最適 な条 件 を,i)DNAの 抽 出法,ii)Bufferの 種 類, iii)PCRの 温度 と回数,お よび,iv)用 いたprimerの 種 類 ご ...
متن کاملInvestigations of genetic variation between olive (Olea europaea L.) cultivars using arbitrarily primed polymerase chain reaction (AP-PCR).
Characterization and selection of olive clones for the production of olive oil is essential in Turkey because of its profitable exploitation. AP-PCR (Arbitrarily-Primed PCR) is a technique that can distinguish the genetic relationship among plant species and other organisms. In this study, AP-PCR approach was used in order to determine the genetic relationship of different six olive clones. The...
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ژورنال
عنوان ژورنال: Journal of the Japanese Association for Infectious Diseases
سال: 2003
ISSN: 1884-569X,0387-5911
DOI: 10.11150/kansenshogakuzasshi1970.77.1040